3D DIY Bio-printer: Bioengineering project

By ML -
3D bio-printers can be used to print biological materials such as gels, proteins and cells. Inspired by the BioCurious team, our group at Nanyang Technological University (NTU) Singapore assembled a 3D DIY bio-printer and explored the possibility of printing mammalian cells.

First, we assembled the printer following the manual from Instructables.








This is the DIY 3D bio-printer created by team at NTU Singapore.





We tested the bio-printer for printing alginate gels. Calcium chloride solution was dropped onto a culture dish containing 1%, 2%, 3% and 4% sodium alginate solution. We found that 1.5-2.0% alginate worked best in providing most consistency in printing. The picture below shows a test print using ink versus alginate hydrogel.

(Left) Letter 'N' printed in ink (Right) Letter 'N' printed in alginate
Scale bar: 1mm

Finally we showed that 3D gels could be printed using this DIY bio-printer platform, the picture below shows a printed gel with 200 layers of printing. And we also printed mammalian cells (HS5 stromal cells) using this DIY 3D bio-printer in alginate hdyrogel.

(Left) Printed 3D gel (Right) Mammalian cells printed with alginate hydrogel
Green - live cells; Red - dead cells 


Acknowledgements: This project was accomplished by Ernesto Irving Rivera Soto, Poon Ling Jun and Yeo Chee Wee, supervised by Drs. Mayasari Lim (SE3D Education) and Xu Chenjie (NTU)

Comments

  1. UnknownMay 13, 2018 at 8:55 AM

    I havent any word to appreciate this post.....Really i am impressed from this post....the person who create this post it was a great human..thanks for shared this with us. alginate

    ReplyDelete

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